Surface characterization, electrochemical properties and in vitro biological properties of Zn-deposited TiO2 nanotube surfaces

In this work, to improve antibacterial, biocompatible and bioactive properties of commercial pure titanium (cp-Ti) for implant applications, the Zn-deposited nanotube surfaces were fabricated on cp-Ti by using combined anodic oxidation (AO) and physical vapor deposition (PVD-TE) methods. Homogenous elemental distributions were observed through all surfaces. Moreover, Zn-deposited surfaces exhibited hydrophobic character while bare Ti surfaces were hydrophilic. Due to the biodegradable behavior of Zn on the nanotube surface, Zn-deposited nanotube surfaces showed higher corrosion current density than bare cp-Ti surface in SBF conditions as expected. In vitro biological properties such as cell viability, ALP activity, protein adsorption, hemolytic activity and antibacterial activity for Gram-positive and Gram-negative bacteria of all surfaces were investigated in detail. Cell viability, ALP activity and antibacterial properties of Zn-deposited nanotube surfaces were significantly improved with respect to bare cp-Ti. Moreover, hemolytic activity and protein adsorption of Zn-deposited nanotube surfaces were decreased. According to these results; a bioactive, biocompatible and antibacterial Zn-deposited nanotube surfaces produced on cp-Ti by using combined AO and PVD techniques can have potential for orthopedic and dental implant applications.

TiO 2 nanotube arrays on Ti-based surfaces are widely investigated for different biomedical applications such as drug delivery, biosensors, cardiovascular stents, dental implants and orthopedic implants [21][22][23] . Osteoinductive potential of TiO 2 nanotube arrays surfaces was improved compared to smooth surfaces. Moreover, bioactivity of these surfaces enhances the adsorption of extracellular proteins such as fibronectin, fibrinogen and vitronectin, which is an essential factor in the early stage of biomaterials implantation 24 . However, these surfaces can be exposed to bacterial colonization under body condition. This can be resulted in losing of implant materials. To prevent bacterial attachment and proliferation on Ti-based surfaces, antibacterial elements such as silver (Ag) [25][26][27][28][29] , copper (Cu) 9,30,31 and zinc (Zn) [32][33][34] can be doped.
Ag and Cu can indicate cytotoxic effects although Ag and Cu possess much better antimicrobial activity than other antibacterial elements against to Gram positive and Gram negative bacteria 35 . Zn can indicate a relatively weak antibacterial activity compared to Ag and Cu. However, Zn is an important trace element in bone tissue possesses low cytotoxic effect 36 . Furthermore, Zn positively influences the bone mineralization and growth. Moreover, its deprivation impairs the bone metabolism 37,38 . In addition, Zn plays many important roles such as DNA synthesis, nucleic acid metabolism, enzyme activity, biomineralization and hormonal activity 39,40 . Thus, Zn has been of aroused interest of the biomedical community at last years.
Several investigations were carried out on Zn-doped, Zn-incorporated or ZnO nanoparticles deposited TiO 2 nanotube on titanium and its alloys at last decade for biomedical applications 17,[41][42][43][44][45][46] 46 . However, according to above cited works, homogeneous and high purity surfaces could not be fabricated on surfaces by the nature of coating processes. Thus, it is essential that homogeneous nano-layer surfaces are produced on metal-or ceramic-based surfaces without any impurity under vacuum conditions by PVD-TE methods in this work.
There is no any report on fabrication, characterization, corrosion and in vitro biological properties (bioactivity, biocompatibility and antibacterial properties) of Zn-nanolayer deposited TiO 2 nanotubes on commercial pure titanium by using combined AO and PVD-TE processes in the literature. In this work, homogeneous and high purity ZnO-based nanotube arrays on cp-Ti surfaces were fabricated by AO and PVD-TE processes for the first time in literature. The present article deals with commercially pure titanium for dental applications, then coated with ZnO-deposited nanotube layers by AO and PVD-TE processes. Initially, nanotube arrays were formed on cp-Ti surfaces by AO process. And then, Zn nanolayers with thickness of 1 nm, 3 nm and 5 nm were deposited on nanotube arrays by PVD-TE process. The surface morphology, elemental distribution, elemental amount, phase structure, topography and wettability of all surfaces were characterized by SEM, EDX-mapping, EDX-area, XRD, AFM and contact angle measurement, respectively. Electrochemical corrosion properties were evaluated under SBF conditions by Tafel extrapolation method. Bacterial adhesions were investigated via Gram-positive such as Streptococcus pyogenes, Staphylococcus aureus, Bacillus subtilis and Gram-negative such as Pseudomonas aeruginosa, Escherichia coli, Salmonella typhimurium microorganisms. In vitro biocompatibility tests such as cytotoxicity, artificial blood adhesion, ALP activity and hemolytic activity were investigated.

Experimental details
Sample preparation. Cp-Ti sheets (ASTM F67 Grade 2; Shaanxi Aone Titanium) were cut the sizes of 10 mm × 10 mm × 1 mm and they were ground up to 2000 # SiC sandpapers as given in Table 1. Then, all polished sheets were cleaned in an ultrasonic bath and were dried by heat gun.
Anodic oxidation (AO) method. Cp-Ti sheets were coated in NH 4 F (0.5% wt.) and deionized water (5.0% vol.) containing ethylene glycol electrolyte below 30 °C with a DC power supply (GW Instek PSU 400) by AO process. The AO process of all sheets was carried out at 50 V for 1 h. During AO, the cp-Ti and Pt sheets were used as anode and cathode, respectively. At post-treatment AO, the heat treatment was applied to AO surfaces at 450 °C for 1 h. Thus, amorphous phases were transformed to crystalline form 47,48 . Physical vapor deposition (PVD-TE) method. The Zn nano layers with 1 nm (Zn-1 nm), 3 nm (Zn-3 nm) and 5 nm (Zn-5 nm) were accumulated onto nanotube arrays by PVD-TE (Vaksis PVD-2T). The vacuum chamber was pumped down until the base pressure reached approximately 1 × 10 -6 mbar. Chamber pressure increased up to 2 × 10 -5 mbar owing to the evaporation of zinc powders during process. The Zn was evaporated by increasing the current gradually up to 30 A. The deposition rate was kept as 0.5 A°/s through Bacterial adhesion test. The antibacterial activity of the coating surfaces was determined by investigating the adhesion of Gram-positive and Gram-negative bacteria. 100 µL of a bacterial culture (0.5 McFarland) was added to the broth medium and 0.5 cm 2 of coating area was transferred to the medium containing the bacteria. After 24 h of incubation at 37 °C, all coatings were washed twice in succession with 15 mL saline solution. Ultrasonic treatment was performed to obtain the bacteria adhering to the surface. 2 mL of physiological solution was applied to the coatings and sonicated at a frequency of 35 kHz for 5 min. After sonication, 25 µL of the sample was cultivated in nutrient agar dishes to ensure re-cultivation, and colonies were counted after incubation.
Cell viability test. The cytotoxic effects of each coatings were determined by MTT assay using lymphoblastoid cells (RPMI 8866 cell line). For cell proliferation a medium containing RPMI 1640, penicillin (100 IU/ mL), fetal bovine serum (10%), glutamine (2 mM) and streptomycin (100 µg/mL) was used. 0.5 cm 2 coating was transferred to cell culture containing 2 × 10 5 cells/mL. The coatings and the cells were incubated for 48 h. Then 5 µg/mL MTT (20 µL) and isopropanol (200 µL) were added to meium. The change in cell viability was determined by spectrophotometric measurement (570 nm) and after 4 h of incubation, the absorbance of the medium was measured. Artificial blood protein adhesion. To determine the hemocompatibility of the coatings, the adhesion of the proteins gamma-globulin, albumin, and fibrinogen was studied. Solutions of blood proteins were prepared at a concentration of 20 mg/mL, and the coating surfaces were incubated with the protein solutions in a batch system for 2 h at 37 °C. After the interaction, a gentle wash with 15 mL of distilled water was performed to remove non-adherent proteins from the surface. The proteins that adhered to the surface were obtained using ultrasonication at a frequency of 35 kHz. The amount of protein separated from the surface after ultrasonication was determined by spectrophotometric measurement (280 nm).
Hemolytic activity (blood compatibility) test. Hemolytic  Statistical analysis. Statistical analysis was performed using "IBM SPSS Statistics 22 SP" and all results are given as mean ± SD. p < 0.05 was considered statistically significant.

Results and discussion
SEM analysis of the surfaces. The morphologies of nanotube and Zn-deposited nanotube surfaces of different thicknesses are given in Fig. 2. There are three stages (I, II, III) for the growth process of TiO 2 nanotubes in the direction of the oxygen bubble model. These are [(i) the formation of oxide layer, (ii) tube initiation, and (iii) well-ordered nanotube growth] are better explained 1 . The ionic current promotes oxide growth. Subsequently, the electronic current produced by oxygen evolution maintains the nanotube growth. The oxide compact layer and the expansion of oxygen bubbles entrapped between electrolyte/oxide interface lead to a deformation of the compact oxide layer. This resulted in forming a hemispherical bottom that acts as a mold for the nanotubes. After the releasing of O 2 gas and oxide layer growth rates are stable, self-organized nanotube growth (stage III) occurs 53,54 . Homogeneous depositions were observed on the Zn-deposited surfaces and the nanotube morphology was completely covered by newly deposited Zn layers as seen in Fig. 2b-d. As a result, the surface chemistry and nanotube surface morphology have changed. Although the pore structure was partially preserved on the surface, the presence of nanotubes could not be preserved. This is due to the difference in the nature of the Zn structure observed during the TB process. The powder Zn metal rapidly adheres to the nanotube surface by spraying directly into the boiler within a short time, without showing melting behavior in the tungsten melting pot at low current values and for long periods. This situation causes morphological changes and laminar growths on nanotube surfaces as seen in Fig. 2. Depending on the increase in the deposition area on the nanotube surfaces, a trend from needle-like morphology to laminar morphology is observed with increasing thickness. Thus, the nanotube morphology is eliminated. EDX analysis of the surfaces. The Ti and O structures that homogeneously distributed throughout the surface were observed in all Zn-deposited samples as seen in Fig. 3. Also, as expected, Zn structures were also observed and homogeneous distribution was detected along the surface due to thermal deposition. However, the Zn signal could be relatively weak for Zn-1 nm and Zn-3 nm layers since e-beam with a 15 kV can penetrate to a depth to 1 µm. In addition, the amounts of Zn-based elements observed on nanotube surfaces are given in the Table 2. During the TB process, depending on the thickness values measured with the crystal thickness monitor integrated into the device, it was determined that the amount of antibacterial Zn accumulated with increasing thickness increased and values below the cytotoxic limit were observed. But as seen in Zn morphology; In particular, a very high rate of Zn was detected in the 5 nm coating. The layer that completely covers the surface in the SEM images and the elemental values support each other. Especially for Zn, coatings below 3 nm can be preferred to preserve the morphology and reduce the cytotoxic limit value to lower levels. After AO process, the nanotube surfaces are transformed from amorphous to crystalline structures without any morphological variations by a diffusion mechanism through 60 min at 450 °C as described in experimental section. In our previous works 48,55,56 , the AO formation mechanisms under the existence of Fwere discussed in detail. The AO process is the electrolysis of H 2 O at initial reactions. Subsequently, a compact TiO 2 layer is occurred by dissolving of F − on substrates. Finally, nano-pits occur at the last reaction transform into nanotube arrays at the suitable voltage, current and time during AO. Furthermore, as seen in Fig. 4b-d, a trace amount of ZnO was detected on the Zn-deposited nanotube surfaces. Already, the existence of elemental Zn structures was verified on all surfaces by EDX-mapping and EDX-area. The Zn powders were evaporated onto the nanotube surfaces under vacuum chamber through PVD-TE process. However, Zn-based nanotube surfaces can be transformed from metallic to ceramic form due to oxygen affinity of zinc under atmospheric conditions at post-fabrication PVD-TE process as shown in Fig. 4. Thus, the presence of bactericidal ZnO on the nanotube surfaces was confirmed by XRD.
Roughness and topography of the surfaces. Average roughness (Sa) results of bare cp-Ti, bare nanotube and Zn-deposited nanotube surfaces are given in Table 3. As can be seen from Table 3, the lowest roughness result was measured at 10.23 nm in the bare cp-Ti. Average roughness of bare nanotube surfaces is higher than one of bare cp-Ti due to existence of hollow nanotube morphology. Average roughness values of Zn-deposited nanotube surfaces; significantly increased compared to bare cp-Ti and bare nanotube surfaces. Compared to cp-Ti, the increase in surface chemistry as well as roughness values after AO and TB processes is one of the parameters that positively affect cell adhesion and tissue growth. The average surface roughness values of the Zndeposited nanolayer coating structures vary between approximately 144-186 nm, respectively. However, average roughness of Zn-1 nm is great compared to Zn-3 nm whereas average roughness of Zn-5 nm is maximum value. These were also supported by surface SEM morphologies as seen in Fig. 2b-d. The surface morphologies on Zn-1 nm, Zn-3 nm and Zn-5 nm refer to lath, needle and planar surface structures, respectively. A bit more rough lath protruding structures appear on Fig. 2b compared to Fig. 2c due to deposition nature of Zn structures through PVD-TE process. This leads to reduce roughness from Zn-1 nm to Zn-3 nm as given in Table 3 since protruding structures on Zn-1 nm are transformed to thin needle-like low projection structures on Zn-3 nm. Moreover, more rough planar protruding structures appear on Fig. 2d compared to Fig. 2b,c due to deposition www.nature.com/scientificreports/ nature of Zn structures through PVD-TE process. This leads to increase roughness from Zn-3 nm to Zn-5 nm as given in Table 3 since thin needle-like low projection structures on Zn-3 nm are transformed to planar protruding structures on Zn-5 nm. As seen in Fig. 5, the AFM topographic images of the Zn-deposited nanotube surfaces support the laminar formation as seen in the SEM images.
Wetting abilities of the surfaces. Mean contact angle measurement results and wetting images of bare cp-Ti and Zn-deposited nanotube surfaces are given in Table 4 and Fig. 6, respectively. Surfaces with low contact angle show hydrophilic character as they can be completely wetted. Surfaces with a high contact angle exhibit hydrophobic character. While the contact angle of the surface is 90° and below, it shows hydrophilic property, while above 90° it shows hydrophobic property. The mean contact angle of the bare cp-Ti was measured as 79.7° ± 0.3. Therefore, cp-Ti shows hydrophilic properties. The mean contact angle measurement values of the Zn-deposited nanolayer coating structures on cp-Ti ranged from approximately 131.3° ± 0.1 to 149.7° ± 0.0, respectively. Bare cp-Ti surfaces generally showed lower wetting angle values than coated surfaces. The wettability of the surfaces depends on the surface energy, roughness, porosity and surface charges. The water droplet first comes into contact with the passive oxide surface on the bare cp-Ti surface, and the surface       59 . Therefore, contact angle values of TiO 2 nanotube arrays are higher than substrate and literature works 59,61,62 . This type of result on TiO 2 nanotube arrays was also observed in our previous study and discussed in detail 56 . Similarly, the water drop touches the Zn nanolayer on the TiO 2 nanotube surface. Although these surfaces are based on nanolayers, the surface chemistry and surface energy have also changed due to the antibacterial structures on the surface. For this reason, just like in AO-based nanotubes, the atmosphere gases inside the tubes create resistance for a short time in the first place and avoid the surface from getting wet. This result points to the Cassie-Baxter situation. According to this; the liquid occur a contact line with the air trapped below the contact line on the rough surface. In addition, Zn-based structures increase the hydrophobic properties of the surface by reducing the polar properties of the surface. This is supported by experimental results. Also, regardless of the nanotube morphology, the Zn-based nanolayer surface structure covered the nanotube surfaces, showing www.nature.com/scientificreports/ morphology ranging from needle-like to laminar surface structures. The Zn-based nanolayer showed mostly hydrophobic properties on the surfaces. Therefore, besides the surface morphology, the chemistry of the surface; it significantly affects the hydrophobic/hydrophilic behaviors of the surface. The hydrophilic/hydrophobic surface affects adhesion and spreading of the cells. While some studies in the literature indicate that cell proliferation is easy on hydrophilic surfaces, some studies have shown that cell adhesion is better on hydrophobic surfaces 63,64 . Moreover, it has been reported in the literature that hydrophobic surfaces absorb proteins more strongly than hydrophilic surfaces 65,66 . In addition, the hydrophobicity of the surface reduces bacterial adhesion and spread. Thus, it contributes antibacterial properties.
Electrochemical corrosion properties of the surfaces. Open circuit potentials (OCP) and Tafel extrapolation results (corrosion current density (i cor ), corrosion potential (E cor ) and polarization resistance (Rp)) of bare cp-Ti and Zn-deposited nanotube surfaces are given in Table 5. Corrosion resistance depends on many parameters such as surface morphology, coating thickness, structural defects and phase structure. Bare cp-Ti metal showed lower corrosion current density by passivation at higher potential values compared to Zn-deposited nanotube surfaces. Thus, bare cp-Ti exhibited higher corrosion resistance. Here, the passive oxide layer on the bare cp-Ti surface causes passivation behavior. Predominantly oxide nanotube structures on the surfaces, the protective oxide layer on the surface creates a barrier between the substrate metal and the atmosphere, reducing the ion release. Moreover, metal nanolayers on the surface are hydrophobic, resulting in increased corrosion resistance. However, due to the biodegradable behavior of Zn on the nanotube surface, Zn-deposited nanotube surfaces showed higher corrosion current density than the bare metal. It has been concluded that the corrosion current density increases and corrosion resistance decreases due to the increase in the amount of Zn with increasing thickness of the Zn nanolayer structure. As a result; although the amount increases with increasing thickness with PVD-TE on nanotube surfaces, the solubility and hydrophilic/hydrophobic properties of the layers dominate the corrosion resistance. For implant applications, the antibacterial Zn structure on the nanotube surface must maintain its presence on the surface until the healing is completed, and then it is necessary for its gradual removal from the surface.
Antibacterial activities of the surfaces. The cidal effect rates of Zn-deposited nanotube surfaces on different bacteria are given in the Fig. 7. Zn accumulation on nanotube surfaces caused a decrease in the number of colonies in bacteria. The highest antibacterial activity for Zn-deposited nanotube surfaces was found to be 62.9-66.6% in Gram-negative bacteria. When all Zn-deposited nanotube surfaces were examined, the highest bacterial inhibition was obtained with 5 nm surface against P. aeruginosa as seen in Fig. 8. In antibacterial studies with 3 nm surface, the highest inhibition was obtained against S. typhimurium, while 1 nm surface showed Table 5. Electrochemical corrosion parameters of bare cp-Ti and Zn-deposited nanotube surfaces.

OCP (V) E cor (V) I cor (A/cm 2 ) Rp (ohm)
Bare Cell viabilities of the surfaces. The change in viability of cells adhering to bare cp-Ti and Zn-deposited nanotube surfaces was investigated by MTT test. It was determined that the viability of cells with adhesion on cp-Ti surfaces was generally decreased compared to the negative control group. Similarly, Kara and Pürçek determined that the viability of the SAOS-2 cell line decreased after interaction with Cp-Ti and Ti-6Al-4 V surfaces 69 . The change in the viability of cells adhering to Gr2 and Zn-deposited nanotube surfaces is given in Fig. 9. It was determined that cell viability was decreased on all Zn-deposited titanium surfaces compared to the negative control. However, it was determined that there was an increase in cell viability on Zn-deposited nanotube surfaces when compared to bare cp-Ti surfaces. This result indicates that titanium deposited Zn on the surface positively affects cell proliferation. As the thickness of Zn deposited on the surface changed, low-level and statistically insignificant changes were detected in cell viability. These changes are due to the effect of nanolayer structures formed after Zn deposition on the surface, affecting cell adhesion. In addition, increasing the Zn layer thickness (from 1 nm to 3) nm on nanotube surface positively affected cell viability. In the literature, Zhao et al. stated that the adhesion, proliferation and viability of osteoblast cells on Zn-deposited rough titanium surfaces are quite high and this situation is related to the nanoparticle surface. They also reported that the surfaces exhibited high antibacterial properties 70 . Contrary to these findings, Ortiz et al. observed a decrease in the viability of cells adhering to Zn apatite coated titanium surfaces compared to the titanium surface 71 .
ALP activities of the surfaces. A profile of ALP activity was obtained parallel to the cell viability data on Zn-deposited nanotube surfaces as shown in Fig. 10. Compared to the negative control, ALP activity was decreased on Zn-deposited nanotube surfaces. When the Zn-deposited nanotube surfaces are compared within themselves, it can be stated that the ALP activity increases depending on the Zn density on the surface. It is known that Zn metal increases protein synthesis and ALP activity in cells, and these increases positively affect cell viability 72,73 . Also, Yang et al. found that ALP activity increased for 14 days in osteoblast cells on Zn-deposited modified titanium surfaces 74 .  Table 6. The protein adsorption tendency of the surfaces was investigated with albumin, globulin and fibrinogen proteins, which are among the major proteins in the blood. In general, a decrease in protein adsorption was observed after Zn coating for all tested proteins. Adsorption of albumin, fibrinogen and globulin to bare cp-Ti surfaces were 19.21 ± 0.53 mg/cm 2 , 17.72 ± 0.85 mg/cm 2 and 14.91 ± 0.29 mg/cm 2 , respectively. After coating the nanotube surfaces with Zn, it was determined that the adsorption of all tested proteins decreased; the lowest adsorption for albumin and fibrinogen was obtained with Zn-3 nm, and for globulin with Zn-5 nm surfaces. However, no statistically significant difference was observed in globulin adsorption on Zn-5 nm and Zn-3 nm surfaces (p > 0.05). No direct correlation was found between protein adsorption and surface-deposited Zn density on all Zn-deposited nanotube surfaces tested. Protein adsorption is closely related to the wettability and roughness of the surface. In addition, surface porosity also plays an important role in protein adsorption by creating a large adsorption area. Increases in   www.nature.com/scientificreports/ surface roughness with surface modification methods cause decreases in protein adsorption 75,76 . Ferraris and Spriano reported that nanotubular structures with high roughness increase protein adsorption, and surfaces with high Zn reduce protein adsorption due to their lower roughness 77 . The albumin, fibrinogen and globulin adhesion test was investigated for blood compatibility. Adhesion of these proteins to the coating surfaces will also trigger the adhesion of blood cells. Adhesion of blood proteins to the surfaces of implants in contact with blood leads to numerous adverse reactions such as coagulation. Low adhesion of blood proteins is an important indicator of hemocompatibility. For this reason, the adhesion of blood proteins to the surface must be tested in the evaluation of blood compatibility. Hemolytic activities of bare cp-Ti and Zn-deposited nanotube surfaces for different periods are given in the Fig. 11. In general, it was determined that the hemolytic activity increased with the increase in time. It was determined that the hemolytic activity after Zn coating processes increased compared to bare cp-Ti surfaces. Bare cp-Ti surfaces exhibited 0.2-0.44% hemolytic activity. The Zn-deposited nanotube surfaces tested between 8, 16 and 24 h showed hemolytic activity in the range of 2.28-0.67%.
Despite these increases observed in hemolytic activity, both bare cp-Ti and Zn-deposited nanotube surfaces are acceptable as surfaces that do not exhibit hemolytic activity. In the literature, biomaterials are classified in three different categories depending on hemolysis: Materials resulting in hemolysis above 5% are classified as hemolytic, between 5 and 2% as mild hemolytic, and below 2% as non-hemolytic 78 . According to this classification, the surfaces obtained by Zn coating processes did not exhibit hemolytic activity and preserved their blood compatible feature.

Conclusions
The present study evaluated the Zn-deposited nanotube surfaces on cp-Ti surfaces using the AO and PVD-TE processes with positive results for biomedical implant applications. The use of the AO and PVD-TE processes induce the formation of homogenous and high purity layers and have the aim of improving antibacterial and biocompatible properties of the dental and orthopedic implants. After Zn-deposition, Zn layers were homogenously covered onto nanotubes. Ti, O and Zn observed on the surfaces were distributed homogenously through the whole surface. Ti, anatase-TiO 2 and bactericidal ZnO phases were obtained on Zn-deposited nanotube surfaces. Zn-deposited nanotube and bare nanotube surfaces exhibited hydrophobic character while bare cp-Ti indicated hydrophilic properties. This is beneficial for antibacterial activities as supported in results. Zn-deposited nanotube surfaces showed higher corrosion current density than the bare metal under SBF conditions. Cell viability, ALP activity and antibacterial properties of Zn-deposited nanotube surfaces were improved with compared to bare cp-Ti. In addition, hemolytic activity and protein adsorption of Zn-deposited nanotube surfaces were reduced. All of these preliminary results suggest a good interaction with living tissue. Thus, the Zn-deposited TiO 2 nanotube coatings of titanium studied in this work offer very promising results for future dental implant applications due to the properties of antibacterial and biocompatible.

Data availability
The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request.